Putative orthologous gene clusters to P. fijiensis PKS10-2, from P. fuligena and P. cruenta.

For each gene in the putative biosynthetic cluster, the table indicates the description in Fig 10, the accession of the putative P. fijiensis homolog protein in cases where they are each other’s best blast hit, the scaffold wherein the P. fuligena or P. cruenta gene is located, the location along the scaffold, the gene orientation, the protein accession of the sequence if one has been annotated, the Interpro and KOG descriptions, and the conserved domains identified in the Conserved Domain Database (CDD) via blastp or blastx, via the CDD v3.18–55570 PSSMs or NCBI_curated—16212 PSSMs databases. Blastx or blastp analysis was done for each gene or protein sequence using the non-redundant protein sequence database on NCBI, and the top ten hits are indicated with species wherein they are found, description of the sequence, bitscore, E-value, percent sequence identity, and accession. If predicted protein sequences were previously annotated, then reciprocal blastp searches were used to identify homologs of P. fijiensis sequences, conserved domains were identified in the CDD database using blastp, and blastp analysis was used to identify the top ten hits from the non-redundant protein sequence database on NCBI. If predicted protein sequences were not previously annotated, then putative genes were identified from the P. fuligena and P. cruenta genomes using tblastn of P. fijiensis cluster genes as queries, blastx was used to verify that they were reciprocal best hits, blastx was used to identify conserved domains, and blastx was used to identify the top ten hits from the non-redundant protein sequence database on NCBI. A) The putative biosynthetic cluster for P. fuligena; B) The putative biosynthetic cluster for P. cruenta. (XLSX)