Impact of miR-199-5p, miR-199a-3p and miR-214-3p overexpression on human lung fibroblasts

To identify putative novel specific targets of miR-199-5p, miR-199a-3p and miR-214-3p, we overexpressed these miRNAs in human MRC5 pulmonary fibroblasts (CCL-171) using synthetic pre-miRNAs or a synthetic “negative” pre-miRNA control (miR-Neg). RNA samples were harvested 48 hours post-transfection and 3 independent experiments were carried out. 3 independent experiments performed in a one color design, corresponding to 4 conditions (miR-Neg, miR-199-5p, miR-199a-3p, and miR-214-3p) for a total of 12 samples.