Induction in fibroblasts of chemokines and growth factors after treatment with single or combination of factors.

Induction of chemokines (CCL2, CCL7 and CCL8) and growth factors (AREG, NRG1,WISP1 and STC1) in HFFF2 fibroblasts co-cultured with breast cancer cells or normal breast epithelial cells (co-culture Cal51; co-culture MDAMB231; MCF10A co-culture) or upon treatment with individual factors IL1β (10 ng/ml), AREG (100 ng/ml) or TGFβ (10 ng/ml) or a combination was measured by qRT-PCR. Fold induction was calculated as the change in gene expression compared to untreated HFFF2 control cells. GAPDH was used as the normalization control for all experiments. Data shown is average of three experiments. Asterisk indicated that the fold induction is significant; p<0.05.