SvevoZavitan_introgression_line_genotyping

The goal of this study was to investigate if the introduction of small wild emmer introgressions can expand the range of phenotypic response to water stress in an elite wheat cultivar background. To accomplish this, we developed a set of introgression lines (ILs) using elite durum wheat cultivar, Svevo as the backcross parent, and Zavitan as the source of wild emmer introgressions (Avni et al., 2014). Zavitan is well-adapted to the semi-arid environment and has a sequenced and annotated genome thus making it more accessible for downstream genetic analysis compared to other wild emmer accessions (Avni et al., 2017). The subset of ILs selected for this study by eliminating the wild alleles for the Reduced height (Rht)-B1b gene and non-brittle spike (TtBtr1) genes. This set included 47 wild emmer ILs were genotyped using the 90K SNP chip, and poses 1.3-14.2% of the Zavitan genes per IL.

本研究旨在探究在优良小麦品种的遗传背景中引入小型野生二粒小麦（wild emmer）渐渗片段，能否拓宽其对水分胁迫的表型响应范围。为达成该研究目标，本研究以优良硬粒小麦（durum wheat）品种Svevo作为回交亲本，以Zavitan作为野生二粒小麦渐渗片段供体，构建了一套渐渗系（introgression lines, ILs）群体（Avni等，2014）。Zavitan对半干旱环境适应性良好，且其基因组已完成测序与注释，相较于其他野生二粒小麦种质材料，更便于开展后续遗传分析（Avni等，2017）。本研究所筛选的渐渗系子集，剔除了携带矮秆（Reduced height, Rht)-B1b基因与不落穗（non-brittle spike, TtBtr1）基因的野生等位基因。该套群体包含47份野生二粒小麦渐渗系，均通过90K SNP芯片（90K SNP chip）完成基因型分型，每份渐渗系携带1.3%~14.2%的Zavitan基因组片段。