Genome-wide expression changes in early wt, Dnmt1−/− and TKO EBs.

Scatter plots of genome-wide expression changes between day 0 and 4 in TKO (A) and Dnmt1−/− EBs (B) relative to wt EBs. Data points are heat-colored according to their local density. About 17% of 25528 analyzed genes changed expression level in wt EBs according to the thresholds indicated by the vertical dotted lines. Genes whose expression levels increased and decreased in wt but not in TKO EBs (“TKO non-responders”, parts of the corridor between the horizontal dotted lines to the left and right of the vertical dotted lines in A) are marked in green in both A and B and their numbers are reported in green in the respective sectors.以野生型（wt, wild type）拟胚体（EBs, embryoid bodies）为参照，展示三重敲除（TKO）样本（图A）与Dnmt1基因敲除纯合子（Dnmt1−/−）样本（图B）在第0天与第4天之间的全基因组表达变化散点图。数据点依据其局部密度以热色编码标注。本研究共分析25528个基因，其中约17%的基因在野生型拟胚体中的表达水平发生了符合垂直虚线所示阈值的变化。仅在野生型拟胚体中出现表达上调或下调、而在三重敲除拟胚体中无表达变化的基因（即图A中垂直虚线左右两侧、水平虚线所围成区间内的"TKO无应答基因（TKO non-responders）"），在图A和图B中均以绿色标记，其基因数量也在对应扇区以绿色字体标注。