Development and evaluation of an indirect ELISA for detection of Teladorsagia circumcincta infection in sheep

The gastrointestinal helminth, <i>Teladorsagia circumcincta</i>, is one of the major health risk and production-limiting disease in small ruminant populations, particularly in temperate regions. With the increasing importance of the disease management and recruited anthelminic resistant types, accurate approaches are needed for diagnosis of the infection in the host. Because of uncertain results using faecal examinations, the ELISA method was indicated for detection of the nematode antigenic materials. Despite some promising results, problems were described in terms of the test specificity and the cross-reactions. The aim of this study was to evaluate worm somatic and excretory/secretory (ES) products using western blot analysis and an indirect ELISA for detecting <i>T. circumcincta</i> infection in sheep. Here, the file total score, includes the OD values and OD rates (ODR) obtained for the somatic and ES materials as well as the positive and negative pool controls.The second file (cross-reaction) shows the values and the graph obtained for the cross-reaction between ES and somatic materials of T. circumcincta and other worms examined. This file was prepared using Graphpad prism software.胃肠道线虫环纹背带线虫（Teladorsagia circumcincta）是威胁小反刍动物种群健康、制约其生产性能的主要病原之一，在温带地区的危害尤为突出。随着该病防控工作的重要性日益凸显，抗蠕虫药耐药虫株不断出现，亟需建立精准的宿主感染诊断方法。传统粪便检测法的结果可靠性欠佳，因此有研究提出采用酶联免疫吸附试验（ELISA）检测线虫抗原物质。尽管已有部分颇具前景的研究结果，但该方法仍存在特异性不足、易发生交叉反应等问题。 本研究旨在通过蛋白质免疫印迹试验（Western Blot）与间接酶联免疫吸附试验，评估环纹背带线虫的虫体体细胞与排泄/分泌（ES）产物，以检测绵羊的该类感染。 本数据集包含两个文件：其一为总评分文件，涵盖了体细胞与ES产物的光密度值（Optical Density, OD）、光密度比值（Optical Density Ratio, ODR），以及阳性与阴性混合对照的相关数据；其二为交叉反应文件，包含环纹背带线虫与其他受试蠕虫的ES产物、体细胞产物间交叉反应的相关数值及对应图表，该文件通过GraphPad Prism软件制作完成。