Assessment of the dormant and proliferative vascular metastatic niche

During metastasis, cancer cells travel the circulation to colonise distant sites. Due to the rarity of these events, the immediate cell fate decisions of metastasising tumour cells (mTC) are poorly understood and the role of the endothelium, as dissemination interface, remains elusive. Using a novel combinatorial mTC enrichment approach, we provide a first transcriptional blueprint of the early colonisation process Mice were injected with niche-labeling dormant (D20R) or proliferative (4T1) breast cancer cell lines and labeled, as well as matched unlabeled lung endothelial cells were sampled 3.5 days post-injection. Comparative gene expression profiling analysis of RNAseq data for lung endothelial cells from the different conditions.