Percentage of enzyme present as PKAc and total PKA activity during freezing and thawing in <i>R. sylvatica</i>.

Values are mean ± SEM (N = 4–8). Total activity of PKA is in U mg−1 protein. Asterisk indicates that the value differs from the mean for the corresponding sample of unfrozen frogs (Dunnett’s, P<0.05); dagger indicates that the value differs between populations (Bonferroni, P<0.05).