Data from: Microsatellite mutation rate in Atlantic sturgeon (Acipenser oxyrinchus)

Understanding mutation rates can greatly extend the utility of population and conservation genetic analyses. Herein we present an estimate of genome-wide microsatellite mutation rate in Atlantic sturgeon (Acipenser oxyrinchus) based on parent-offspring transmission patterns. We screened 307 individuals for parentage and mutation-rate analysis applying 43 variable markers. Out of 13,228 allele transfers, 11 mutations were detected, producing a mutation rate of 8.3x10-4 per locus per generation (95%CI: 1.48x10-3, 4.15x10-4). Single-step mutations predominated and there were trends toward mutations in loci with greater polymorphism and allele length. Two of the detected mutations were most probably cluster mutations, being identified in 12 and 28 sibs, respectively. Finally, we observed evidences of polyploidy based on the sporadic presence of 3 or 4 alleles per locus in the genotyped individuals, supporting previous reports of incomplete diploidization in Atlantic sturgeon.

明晰突变率可极大拓展种群遗传学与保护遗传学分析的应用价值。本研究基于亲代-子代传递模式，估算了大西洋鲟（Acipenser oxyrinchus）全基因组范围内的微卫星（microsatellite）突变率。本研究选用43个多态性标记，对307个个体开展亲权鉴定与突变率分析。在总计13228次等位基因传递事件中，共检测到11处突变，得到的突变率为每位点每世代8.3×10^-4（95%置信区间：1.48×10^-3，4.15×10^-4）。单步突变占主导地位，且突变更易发生在多态性更高、等位基因长度更长的位点。在检测到的突变中，有2处极有可能为簇状突变，分别在12个和28个同胞个体中被检出。最后，本研究在基因分型个体中观察到个别位点存在3个或4个等位基因的零星分布现象，由此获得多倍体相关证据，佐证了此前关于大西洋鲟存在不完全二倍体化的研究报道。