Transcriptome analysis of Prdm11 knockout mice after OVA challenge

PRDM family members encode for proteins functionally associated with the control of cell proliferation, differentiation as well as apoptosis action in cell and tissue-specific menner. As important factors in maintenance and differentiation of human and mouse ES cells several PRDM family members were identified. Prdm11 has an outsider position within the PRDM family due to the lack of zinc-finger domains. However, a zinc-finger binding motive is present and likely assume the function of protein-protein interaction. Prdm11 was described as a candidate for tumor suppressor. However, the function of this gene is still unknown. Our study give evidence a new functional association of Prdm11 in allergic disease and asthma. Total RNA obtained from 1/2 lung of 4 female mice of each analysed group (widltype, wildtype challenged, mutant, mutant challenged)PRDM家族成员编码的蛋白质，其功能涉及以细胞和组织特异性方式调控细胞增殖、分化及细胞凋亡。作为维持人和小鼠胚胎干细胞（ES cells）自我更新与分化的关键因子，多个PRDM家族成员已被鉴定出来。由于缺乏锌指结构域（zinc-finger domains），Prdm11在PRDM家族中具有独特地位。不过该蛋白存在锌指结合基序（zinc-finger binding motif），推测其可介导蛋白质-蛋白质相互作用。Prdm11曾被报道为肿瘤抑制因子候选基因，但其具体功能至今仍未明确。本研究证实了Prdm11与过敏性疾病及哮喘（asthma）之间存在全新的功能关联。本研究的实验材料取自各分析组（野生型组、野生型致敏攻击组、突变型组、突变型致敏攻击组）中4只雌性小鼠的半侧肺组织提取的总RNA（total RNA）。