The transcription factor IRF4 regulates the differentiation and function of intestinal ILC3s.

To investigate the role of IRF4 in the regulation of ILC3 development and function, we used the IRF4flox/floxRorccre mice and C57BL/6 mice(6-8 weeks-old ) to carry out single-cell RNA sequencing (BD Rhapsody) on gut ILC3s. ILC3s from IRF4flox/floxRorccre mice (6-8 weeks-old ) and control C57BL/6 mice (6-8 weeks-old ) are isolated from the small intestine and analyzed by single-cell RNA sequencing technology (BD Rhapsody).