Effect of dentin moisture on the adhesive properties of luting fiber posts using adhesive strategies

Abstract The purpose of this study was to evaluate the effect of dentin moisture (moist and dry) on the bonding of fiber posts to root dentin with different adhesive strategies (etch-and-rinse, self-etch, and self-adhesive). Seventy-two extracted single-rooted human teeth were endodontically treated and divided into six groups (n = 12) according to the moisture of dentin surface and adhesive systems as follows: a) etch-and-rinse/moist, b) etch-and-rinse/dry, c) self-etch/moist, d) self-etch/dry, e) self-adhesive/moist, and 6) self-adhesive/dry. The specimens were sectioned into six slices for push-out bond strength (BS), nanoleakage (NL) by SEM, and Vickers microhardness (VHN) of the resin cement. A universal testing machine (AG-I, Shimadzu Autograph) was used at a crosshead speed of 0.5 mm/min until post extrusion, with a load cell of 50 kg for evaluation of the push-out strength. Data on BS, NL, and VHN were evaluated by two-way ANOVA and Tukey’s test (α = 0.05). Dentin moisture as the main factor was not significantly different for the push-out test. However, higher BS values can be observed for the etch-and-rinse group. A lower percentage of NL was found in the dry dentin groups. The moisture pattern was not significant in the hardness values for the pre-etching groups. Additional moisture did not increase the evaluated properties.

摘要 本研究旨在评估牙本质（dentin）湿度（湿润与干燥状态）对不同粘接策略（酸蚀-冲洗（etch-and-rinse）、自酸蚀（self-etch）及自粘接（self-adhesive））下纤维桩（fiber posts）与牙根牙本质（root dentin）粘接效果的影响。选取72颗拔除的单根人牙，完成根管治疗后，根据牙本质表面湿度与粘接系统分为6组（n=12）：a）酸蚀-冲洗/湿润组、b）酸蚀-冲洗/干燥组、c）自酸蚀/湿润组、d）自酸蚀/干燥组、e）自粘接/湿润组、f）自粘接/干燥组。将所有试件切割为6片，分别用于推出粘接强度（push-out bond strength, BS）检测、扫描电镜（SEM）下的纳米渗漏（nanoleakage, NL）观察以及树脂水门汀（resin cement）的维氏显微硬度（Vickers microhardness, VHN）检测。采用岛津Autograph AG-I型万能试验机，以0.5 mm/min的十字头速度加载至桩体脱出，使用50 kg量程的测力传感器（load cell）评估推出粘接强度。对粘接强度、纳米渗漏及维氏显微硬度的数据采用双因素方差分析（two-way ANOVA）与Tukey检验（Tukey’s test）进行统计学分析（α=0.05）。结果显示，作为核心影响因素的牙本质湿度对推出粘接强度无显著影响，但酸蚀-冲洗组的粘接强度值更高；干燥牙本质组的纳米渗漏百分比更低；在预酸蚀组中，湿度对硬度值无显著影响。额外的湿润处理并未提升本研究评估的各项性能指标。