The Antimicrobial Peptide AMP-17 Derived from Musca do-mestica Inhibits Biofilm Formation and Eradiates Mature Bio-film in Candida Albicans

In the current work, we aimed to investigate the antibiofilm of AMP-17 in C.albicans and explored the underlying mechanism. Antifungal susceptibility assay showed AMP-17 exerted a strong in-hibitory efficacy on both biofilm formation and preformed biofilms in C.albicans. RNA-seq was applied to elucidate the underlying molecular mechanism. RNA-seq results showed that MAPK pathway, biosynthesis of antibiotics and essential components of cell were mainly enriched in the biofilm forming stage, while citrate cycle (TCA cycle), phenyla-mine metabolism, propanoate metabolism enriched after biofilm mature. Moreover, the co-expressed DEGs in the two pairwise highlighted the terms of transmembrane transporter activity, regulation of filamentation and biofilm formation acted an important role in the antibiofilm effect of AMP-17. Additionally, qRT-PCR confirmed the level of the genes involved cell adhesion, fila-mentous growth, MAPK, biofilm matrix and cells dispersal correspondingly changed after AMP-17 treatment. Our fundings reveal the underlying antibiofilm mechanisms of AMPs in C.albicans, providing the transcriptional profile of biofilms to open an interesting perspective for development of effective antifungal agents with antibiofilm efficacy in Candida.spp.