RNA pol II ChIP on chip (RPCC)

Adaptation of the ChIP-on-chip protocol, to calculate genomic transcription rates in S. cerevisiae. Keywords: ChIP-chip There are 3 different experimental conditions: -Yeast cell growing exponentially in YPD. -Yeast cell stopped after 2 h of changing them to YPGal. -Yeast cell growing exponentially after 14.5 h of changing them to YPGal. We have used 3 different IP protocols: -total RNA pol II using a Myc tagged RNA pol (RPB1-Myc) -RNA pol II CTD using the Ab 8WG16 (Covance) -RNA pol II CTD Phosphorylated on Ser5 (David Bentley\'s lab) There are 3 independent biological replicates of each experiment.