Calyx growth of Tethocyathus endesa from a crosstransplant experiment carried out in Comau Fjord, Chile

A crosstransplantation experiment between sites of high (Liliguapi) and low pH (XHuinayN) was started in 2014 with specimen of the scleractinian coral Tethocyathus endesa. After the initial collection in 2014 the corals were glued onto polyvinylchloride screws in order to be attached to holders that were installed at the collection sites. Originally 20 corals were collected at each site of which 10 were reinstalled at the same site and 10 were reinstalled at the respective other site. To measure the calyx growth from 2014 to 2016, the calyx was photographed in both years using a camera (OLYMPUS digital camera OM-D E-M5, Olympus Corporation, Tokyo, Japan) with a waterproof housing (NA-EM5 Underwater Camera Housing, Nauticam International Ltd., Hong Kong) that was submerged in a black plastic tub. The tub was filled with filtered seawater from the aquarium in which the corals were held during the laboratory work at the Huinay Scientific Field Station. The corals were photographed next to a sliding calliper functioning as scale. The calyx surface area was then measured and calculated from the photos using Photoshop CS6 (Adobe Systems Incorporated, San Jose, USA) and the results from both years were used to calculate the increase in calyx surface area in 2015 - 2016. […]

2014年，针对高pH位点（利利瓜皮，Liliguapi）与低pH位点（XHuinayN）开展了一项石珊瑚（scleractinian coral）交叉移植实验，实验对象为特西杯珊瑚（Tethocyathus endesa）的采集样本。2014年首次完成样本采集后，研究人员将珊瑚黏附于聚氯乙烯（polyvinylchloride, PVC）螺钉之上，以固定于安装在各采集位点的支架上。本次实验最初在每个位点采集20株珊瑚，其中10株被重新安置至原采集位点，剩余10株则被移植至另一对应位点。为测定2014年至2016年的珊瑚杯生长情况，研究人员于两年间均使用搭载防水罩（NA-EM5水下相机罩，Nauticam International Ltd.，中国香港）的奥林巴斯OM-D E-M5数码相机（OLYMPUS digital camera OM-D E-M5，奥林巴斯株式会社，日本东京）进行拍摄，拍摄设备被置于浸没于黑色塑料水箱的水下环境中。该水箱内灌注有取自惠奈科学野外站（Huinay Scientific Field Station）实验室饲养珊瑚所用水族箱的过滤海水。拍摄过程中，珊瑚旁放置游标卡尺作为比例尺。随后，研究人员利用Photoshop CS6（Adobe Systems Incorporated，美国圣何塞）对照片进行分析，测量并计算珊瑚杯的表面积，并基于两年的测量结果计算2015至2016年间珊瑚杯表面积的增长量。[...]