PacBio fasta files - chr 2 Atlantic cod

79 breakpoint regions witin chromsome 2 of the Atltantic cod was sequenced using HiFi-sequencing, which creates accurate long reads known as HiFi reads. In doing so, I could use multiple, accurate, HiFi sequencing reads to (1) align each breakpoint read to the gadmor3 and coastal reference genome and then (2) make de novo haplotype assemblies for the breakpoint regions for different individuals with the inverted and non-inverted allele. The haplotype assemblies were used in a multiple sequence alignment, and I could pinpoint where the breakpoints are and that they are conserved and fixed within and between populations.<br><br>Here I´ve uploaded the fasta files that were used in making de novo haplotype assemblies.本研究采用HiFi测序（HiFi-sequencing）对大西洋鳕鱼（Atlantic cod）2号染色体上的79个断点区域开展测序，该技术可生成精度优异的长读长序列，即HiFi读段（HiFi reads）。 基于该测序数据，本研究借助多条高精度HiFi测序读段完成两项分析：(1) 将各断点读段比对至GadMor3参考基因组与沿海种群参考基因组；(2) 为携带倒位等位基因与非倒位等位基因的不同个体的断点区域构建从头单倍型组装（de novo haplotype assemblies）。 将上述单倍型组装结果应用于多序列比对后，可精准定位断点位置，并证实这些断点在种群内部及种群间均呈现保守性且已达到固定状态。 本次上传的FASTA格式文件（FASTA files）即为构建从头单倍型组装所用的序列文件。