Transcriptome comparison of oocytes obtained from in vitro culture and in vivo

The comparison of trancriptomes was part of the study by Pfender, Kuznetsov, Pasternak et al, titled: "Live imaging RNAi screen reveals genes essential for meiosis in mammalian oocytes". The goal was to check if the oocytes cultured in vitro in follicles (for RNAi studies) correspond to real gametes obtained directly from mice (in vivo). Apart from functional experiments showing that they can be fertilized and develop into an embryo, we also compared transcriptomes of those oocytes. 3 samples of 50 oocytes were collected for both groups of in vitro and in vivo grown oocytes.转录组（transcriptome）比较是Pfender、Kuznetsov、Pasternak等人开展的研究的一部分内容，该研究的标题为《活细胞成像RNA干扰（RNAi）筛选揭示哺乳动物卵母细胞减数分裂必需基因》。本研究的目标为验证：在卵泡中体外培养（用于RNAi实验）的卵母细胞，是否与直接从小鼠体内获取的天然配子一致。除了通过功能实验证实此类卵母细胞可被受精并发育为胚胎之外，研究团队还对这两类卵母细胞的转录组进行了比较分析。体外培养组与体内发育组卵母细胞各收集3份样本，每份样本包含50枚卵母细胞。