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Data presented in Brinkmann et al., 2021, Aquat. Toxicol.

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These files include all data presented in: Brinkmann et al., 2021. Adsorption of titanium dioxide nanoparticles onto zebrafish eggs affects colonizing microbiota. Aquatic Toxicology. 16SrRNA_(...) fasta and fastq formatted 16SrRNA sequences (Fig. 3c/ Table S4) with in the description line: * Colony numbers corresponding to the numbers included in Table S4 * The applied sequencing primer (27F) * NP exposure: nTiO2 (5 mg/L) or Control (no NPs) * A code referring to the parental zebrafish family that was crossed to obtain the larvae (A7, A8, or A9) 2photon_(...) * Two-photon scans of Fig. 2 * ROI sets of Fig. 2b/e (zipped) * Wavelength and focus information (.dat files) CLSM_(...) * CLSM scans of Fig. 3b (zipped) * ROI (zipped) * Metadata, where scanID corresponds to scan and ROI numbers of the zipped data CFUcounts_(...) CFU count data (Fig. 4/ Fig. S7): * Compound: Exposure: nTiO2 (5 mg/L), nAg (0.25 mg Ag/L), or control (no NPs) * LifeStage: Analyzed life stage: Embryo (1 dpf) or Larvae (5 dpf) * Date: Date of CFU isolation * DilutionFactor: Counted dilution * CFU_Larvae: CFU count per egg or larva DLS_(...) DLS measurements for nTiO2 (Table 2): * Time: Incubation time (h) * Concentration: Nominal concentration (mg/L) * ZP: Zeta potential (mV) * d_size: Z-averag size (nm) DoseResponses_(...) Mortality data of zebrafish larvae exposed to nTiO2 from 3-5 dpf (Fig. S6): * Concentration: Nominal exposure concentration (mg/L) * Day: Exposure time (days). * Mortality: Percentage of larvae that had died * Date: The date at which the mortality was scored EcoPlate_(...) EcoPlate measurement data (Fig. 5/ S3/ S8/ S9): * Compound: Zebrafish egg exposure: nTiO2 (5 mg/L), nAg (0.25 mg Ag/L), or Control (no NPs) * LifeStage: Analyzed life stage: Embryo (1 dpf) or Larvae (5 dpf) * Time: Incubation time (h) * Date: Date of the measurement * EcoPlate: Number of the EcoPlate * Measurement: Measurement number (3 measurements per plate) * 32 substrates (final 32 columns): OD at 590 nm measured for the given substrate PIXE_(...) PIXE measurements (Fig. 1/ Table S2): * ExposureConcentration: Nominal concentration of nTiO2 (mg/L) * Replicate: Replicate number * EggCount: Number of eggs that was analyzed * Date: Date at which the sample was taken * mL_EggWater: Volume of egg water (mL) that was analyzed along with the eggs * Element: Measured element * Total_ppm: Elemental concentration (ppm) * LOD_ppm: LOD (ppm) * ng_Element_per_egg: Elemental concentration (ng/egg) * LOD_ng_per_egg: LOD (ng/egg) TiO2Sedimentation_(...) ICP-OES measurements of Ti for sedimentation analyses (Fig. S4): * Time: Incubation time (h) * ExposureTiO2: Nominal concentration of nTiO2 (mg/L) * MediaTi: Measured concentration of Ti (mg/L) * MediaTiO2: Measured concentration of TiO2 (mg/L) UVspectra_(...) UV-light spectra (Fig. S1): * wavelength: Measured wavelength (nm) * irradiance: Measured irradiance (uW/cm2/nm) * spectrum: Number of the acquired spectrum

本数据集包含Brinkmann等人2021年发表论文中的全部呈现数据:Brinkmann et al., 2021. Adsorption of titanium dioxide nanoparticles onto zebrafish eggs affects colonizing microbiota. Aquatic Toxicology. 即《二氧化钛纳米颗粒吸附于斑马鱼卵会影响定殖微生物群》,刊载于《水生毒理学》(Aquatic Toxicology)。 ### 16S核糖体RNA(16S rRNA)相关数据集 采用fasta与fastq格式存储的16S核糖体RNA序列(对应图3c/附表S4),序列描述行包含以下信息: * 与附表S4中编号对应的菌落编号 * 所用测序引物(27F) * 纳米颗粒暴露处理:nTiO₂(5 mg/L)或对照组(无纳米颗粒) * 用于获得幼鱼的亲代斑马鱼交配家系代码(A7、A8或A9) ### 双光子成像相关数据集 * 对应图2的双光子扫描图像 * 图2b/e的感兴趣区域(ROI)集(已压缩打包) * 波长与焦距信息文件(.dat格式) ### 共聚焦激光扫描显微镜(CLSM)相关数据集 * 对应图3b的CLSM扫描图像(已压缩打包) * 感兴趣区域(ROI,已压缩打包) * 元数据,其中scanID对应压缩数据的扫描编号与ROI编号 ### 菌落形成单位(CFU)计数相关数据集 CFU计数数据(对应图4/图S7): * 暴露化合物:nTiO₂(5 mg/L)、nAg(0.25 mg Ag/L)或对照组(无纳米颗粒) * 生命阶段:分析样本的发育阶段:胚胎(受精后1天,1 dpf)或幼鱼(受精后5天,5 dpf) * 日期:CFU分离日期 * 稀释因子:计数时采用的稀释倍数 * CFU_Larvae:单颗卵或单尾幼鱼的CFU计数 ### 动态光散射(DLS)相关数据集 针对nTiO₂的DLS测量数据(对应表2): * 时间:孵育时长(h) * 浓度:标称暴露浓度(mg/L) * ZP:Zeta电位(mV) * d_size:Z平均粒径(nm) ### 剂量反应相关数据集 斑马鱼幼鱼于3至5 dpf暴露于nTiO₂的死亡率数据(对应图S6): * 浓度:标称暴露浓度(mg/L) * 天数:暴露时长(天) * 死亡率:死亡幼鱼所占百分比 * 日期:死亡率统计日期 ### EcoPlate 测量相关数据集 EcoPlate测量数据(对应图5/图S3/图S8/图S9): * 暴露化合物:斑马鱼卵暴露处理:nTiO₂(5 mg/L)、nAg(0.25 mg Ag/L)或对照组(无纳米颗粒) * 生命阶段:分析样本的发育阶段:胚胎(1 dpf)或幼鱼(5 dpf) * 时间:孵育时长(h) * 日期:测量日期 * EcoPlate:EcoPlate编号 * 测量次数:每块板的重复测量次数(共3次) * 32种底物(对应最终32列):对应底物在590 nm处的光密度(OD)值 ### 质子诱导X射线发射(PIXE)相关数据集 PIXE测量数据(对应图1/附表S2): * 暴露浓度:nTiO₂的标称浓度(mg/L) * 重复次数:重复实验编号 * 卵数量:分析的卵的总数 * 日期:样本采集日期 * mL_EggWater:与卵一同分析的卵水体积(mL) * 元素:被测元素种类 * 总_ppm:元素浓度(ppm) * LOD_ppm:检出限(ppm) * ng_Element_per_egg:单颗卵的元素含量(ng/egg) * LOD_ng_per_egg:单颗卵的检出限(ng/egg) ### 二氧化钛沉降相关数据集 用于沉降分析的钛(Ti)电感耦合等离子体发射光谱(ICP-OES)测量数据(对应图S4): * 时间:孵育时长(h) * ExposureTiO2:nTiO₂的标称浓度(mg/L) * MediaTi:测得的钛(Ti)浓度(mg/L) * MediaTiO2:测得的二氧化钛(TiO₂)浓度(mg/L) ### 紫外光谱相关数据集 紫外光谱数据(对应图S1): * 波长:测量波长(nm) * 辐照度:测得的辐照度(μW/cm²/nm) * 光谱编号:获取的光谱序号
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2021-01-25
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