Data presented in Brinkmann et al., 2021, Aquat. Toxicol.
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资源简介:
These files include all data presented in:
Brinkmann et al., 2021. Adsorption of titanium dioxide nanoparticles onto zebrafish eggs affects colonizing microbiota. Aquatic Toxicology.
16SrRNA_(...)
fasta and fastq formatted 16SrRNA sequences (Fig. 3c/ Table S4) with in the description line:
* Colony numbers corresponding to the numbers included in Table S4
* The applied sequencing primer (27F)
* NP exposure: nTiO2 (5 mg/L) or Control (no NPs)
* A code referring to the parental zebrafish family that was crossed to obtain the larvae (A7, A8, or A9)
2photon_(...)
* Two-photon scans of Fig. 2
* ROI sets of Fig. 2b/e (zipped)
* Wavelength and focus information (.dat files)
CLSM_(...)
* CLSM scans of Fig. 3b (zipped)
* ROI (zipped)
* Metadata, where scanID corresponds to scan and ROI numbers of the zipped data
CFUcounts_(...)
CFU count data (Fig. 4/ Fig. S7):
* Compound: Exposure: nTiO2 (5 mg/L), nAg (0.25 mg Ag/L), or control (no NPs)
* LifeStage: Analyzed life stage: Embryo (1 dpf) or Larvae (5 dpf)
* Date: Date of CFU isolation
* DilutionFactor: Counted dilution
* CFU_Larvae: CFU count per egg or larva
DLS_(...)
DLS measurements for nTiO2 (Table 2):
* Time: Incubation time (h)
* Concentration: Nominal concentration (mg/L)
* ZP: Zeta potential (mV)
* d_size: Z-averag size (nm)
DoseResponses_(...)
Mortality data of zebrafish larvae exposed to nTiO2 from 3-5 dpf (Fig. S6):
* Concentration: Nominal exposure concentration (mg/L)
* Day: Exposure time (days).
* Mortality: Percentage of larvae that had died
* Date: The date at which the mortality was scored
EcoPlate_(...)
EcoPlate measurement data (Fig. 5/ S3/ S8/ S9):
* Compound: Zebrafish egg exposure: nTiO2 (5 mg/L), nAg (0.25 mg Ag/L), or Control (no NPs)
* LifeStage: Analyzed life stage: Embryo (1 dpf) or Larvae (5 dpf)
* Time: Incubation time (h)
* Date: Date of the measurement
* EcoPlate: Number of the EcoPlate
* Measurement: Measurement number (3 measurements per plate)
* 32 substrates (final 32 columns): OD at 590 nm measured for the given substrate
PIXE_(...)
PIXE measurements (Fig. 1/ Table S2):
* ExposureConcentration: Nominal concentration of nTiO2 (mg/L)
* Replicate: Replicate number
* EggCount: Number of eggs that was analyzed
* Date: Date at which the sample was taken
* mL_EggWater: Volume of egg water (mL) that was analyzed along with the eggs
* Element: Measured element
* Total_ppm: Elemental concentration (ppm)
* LOD_ppm: LOD (ppm)
* ng_Element_per_egg: Elemental concentration (ng/egg)
* LOD_ng_per_egg: LOD (ng/egg)
TiO2Sedimentation_(...)
ICP-OES measurements of Ti for sedimentation analyses (Fig. S4):
* Time: Incubation time (h)
* ExposureTiO2: Nominal concentration of nTiO2 (mg/L)
* MediaTi: Measured concentration of Ti (mg/L)
* MediaTiO2: Measured concentration of TiO2 (mg/L)
UVspectra_(...)
UV-light spectra (Fig. S1):
* wavelength: Measured wavelength (nm)
* irradiance: Measured irradiance (uW/cm2/nm)
* spectrum: Number of the acquired spectrum
本数据集包含Brinkmann等人2021年发表论文中的全部呈现数据:Brinkmann et al., 2021. Adsorption of titanium dioxide nanoparticles onto zebrafish eggs affects colonizing microbiota. Aquatic Toxicology. 即《二氧化钛纳米颗粒吸附于斑马鱼卵会影响定殖微生物群》,刊载于《水生毒理学》(Aquatic Toxicology)。
### 16S核糖体RNA(16S rRNA)相关数据集
采用fasta与fastq格式存储的16S核糖体RNA序列(对应图3c/附表S4),序列描述行包含以下信息:
* 与附表S4中编号对应的菌落编号
* 所用测序引物(27F)
* 纳米颗粒暴露处理:nTiO₂(5 mg/L)或对照组(无纳米颗粒)
* 用于获得幼鱼的亲代斑马鱼交配家系代码(A7、A8或A9)
### 双光子成像相关数据集
* 对应图2的双光子扫描图像
* 图2b/e的感兴趣区域(ROI)集(已压缩打包)
* 波长与焦距信息文件(.dat格式)
### 共聚焦激光扫描显微镜(CLSM)相关数据集
* 对应图3b的CLSM扫描图像(已压缩打包)
* 感兴趣区域(ROI,已压缩打包)
* 元数据,其中scanID对应压缩数据的扫描编号与ROI编号
### 菌落形成单位(CFU)计数相关数据集
CFU计数数据(对应图4/图S7):
* 暴露化合物:nTiO₂(5 mg/L)、nAg(0.25 mg Ag/L)或对照组(无纳米颗粒)
* 生命阶段:分析样本的发育阶段:胚胎(受精后1天,1 dpf)或幼鱼(受精后5天,5 dpf)
* 日期:CFU分离日期
* 稀释因子:计数时采用的稀释倍数
* CFU_Larvae:单颗卵或单尾幼鱼的CFU计数
### 动态光散射(DLS)相关数据集
针对nTiO₂的DLS测量数据(对应表2):
* 时间:孵育时长(h)
* 浓度:标称暴露浓度(mg/L)
* ZP:Zeta电位(mV)
* d_size:Z平均粒径(nm)
### 剂量反应相关数据集
斑马鱼幼鱼于3至5 dpf暴露于nTiO₂的死亡率数据(对应图S6):
* 浓度:标称暴露浓度(mg/L)
* 天数:暴露时长(天)
* 死亡率:死亡幼鱼所占百分比
* 日期:死亡率统计日期
### EcoPlate 测量相关数据集
EcoPlate测量数据(对应图5/图S3/图S8/图S9):
* 暴露化合物:斑马鱼卵暴露处理:nTiO₂(5 mg/L)、nAg(0.25 mg Ag/L)或对照组(无纳米颗粒)
* 生命阶段:分析样本的发育阶段:胚胎(1 dpf)或幼鱼(5 dpf)
* 时间:孵育时长(h)
* 日期:测量日期
* EcoPlate:EcoPlate编号
* 测量次数:每块板的重复测量次数(共3次)
* 32种底物(对应最终32列):对应底物在590 nm处的光密度(OD)值
### 质子诱导X射线发射(PIXE)相关数据集
PIXE测量数据(对应图1/附表S2):
* 暴露浓度:nTiO₂的标称浓度(mg/L)
* 重复次数:重复实验编号
* 卵数量:分析的卵的总数
* 日期:样本采集日期
* mL_EggWater:与卵一同分析的卵水体积(mL)
* 元素:被测元素种类
* 总_ppm:元素浓度(ppm)
* LOD_ppm:检出限(ppm)
* ng_Element_per_egg:单颗卵的元素含量(ng/egg)
* LOD_ng_per_egg:单颗卵的检出限(ng/egg)
### 二氧化钛沉降相关数据集
用于沉降分析的钛(Ti)电感耦合等离子体发射光谱(ICP-OES)测量数据(对应图S4):
* 时间:孵育时长(h)
* ExposureTiO2:nTiO₂的标称浓度(mg/L)
* MediaTi:测得的钛(Ti)浓度(mg/L)
* MediaTiO2:测得的二氧化钛(TiO₂)浓度(mg/L)
### 紫外光谱相关数据集
紫外光谱数据(对应图S1):
* 波长:测量波长(nm)
* 辐照度:测得的辐照度(μW/cm²/nm)
* 光谱编号:获取的光谱序号
创建时间:
2021-01-25



