Design of perfused PTFE vessel-like constructs for in vitro applications

The design of a vessel-like construct for further use in perfused tissue models using PTFE membranes and collagen is presented. Endothelial cells were able to adhere to the tube’s wall, resisted perfusion, and formed an endothelial barrier delaying diffusion of fluorescently labeled molecules.本研究提出了一种基于聚四氟乙烯（PTFE）膜与胶原蛋白构建的血管样结构体设计方案，可用于灌注型组织模型的后续研究。实验结果表明，内皮细胞可黏附于该管状结构体的管壁，耐受灌注流体冲击，并形成可延缓荧光标记分子扩散的内皮屏障。