Disruption of neonatal Purkinje cell function underlies injury-related learning deficits
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This folder contains data related to the paper: <strong>Disruption of neonatal Purkinje cell function underlies injury-related learning deficits</strong> Aaron Sathyanesan<sup>1</sup>*, Panagiotis Kratimenos<sup>1,2,3</sup>, and Vittorio Gallo<sup>1,3</sup>*<br> <sup>1</sup> Center for Neuroscience Research and <sup>2</sup> Division of Neonatology, Children’s National Research Institute, Children’s National Hospital, Washington DC<br> <sup>3</sup> George Washington University School of Medicine and Health Sciences, Washington DC<br> * Co-corresponding authorship<br> Email: vgallo@childrensnational.org (Vittorio Gallo)<br> asathyanesan@childrensnational.org (Aaron Sathyanesan) Description of files:<br> 'Figure1.mat' - Contains data related to representative fiber photometry trace in Figure 1D<br> 't' - total time of recording (in seconds)<br> 'dFF_480' - GCaMP6f dFF trace<br> 'dFF_405' - Isosbestic control trace<br> 'USonly', 'CSonly', 'Paired', and 'trials' - categorized time-marked events 'Figure2_and_SIAppendix_FiguresS4-S6.mat' - Contains data related to Figure 2A-D, and SI Appendix Figures S4-S6<br> 'FP_1' - structural array containing relevant data<br> 'FP_1.condition' - 'Nx' or 'Hx' (Figure 2C-D)<br> 'FP_1.day' - learning session 1, 2, or 3 (Figure 2C-D)<br> 'FP_1.zCSUS' - mean z-score dF/F in CS-US window for paired learning trials (Figure 2G)<br> 'FP_1.zdFF' - all z-score dF/F traces (Figure 2C-D)<br> 'FP_1.CSUS_t' - time vector with t = 0 as C.S. onset (Figure 2C-D)<br> 'FP_1.rep_cond' - 'Nx' or 'Hx' for representative data (Figure 2A-B)<br> 'FP_1.rep_day' - learning session 1, 2, or 3 (Figure 2A-B)<br> 'FP_1.rep_dFF' - normalized GCaMP6f dF/F for (Figure 2A-B)<br> 'FP_1.trialduration' - trial durations for all trials (SI Appendix Figure S4)<br> 'FP_1.missteptime' - missteptime for all trials (SI Appendix Figure S5)<br> 'FP_1.velocity' - velocity for all trials (SI Appendix Figure S6)<br> 'FP_1.shortsteptime' - short steptime for trials (SI Appendix Figure S4)<br> 'FP_1.shortmissteptime' - short missteptime for all trials (SI Appendix Figure S5)<br> 'FP_1.longsteptime' - long steptime for trials (SI Appendix Figure S4)<br> 'FP_1.longmissteptime' - long missteptime for all trials (SI Appendix Figure S5)<br> 'FP_1.zPost' - z-scored post-perturbation steptime (Figure 2G) 'Figure3.mat' - Contains data related to Figure 3C<br> 'FP_2' - structural array containing relevant data<br> 'FP_2.condition' - 'saline' or 'CNO'<br> 'FP_2.day' - learning session 1, 2, or 3<br> 'FP_2.zdFF_DRD' - all z-score dF/F traces<br> 'FP_2.CSUS_tmod' - time vector with t = 0 as C.S. onset 'SIAppendix_FigureS2.mat' - Contains data related to SI Appendix Figure S2b-c<br> 'Nx' - structural array containing relevant data to Nx animal<br> 'Hx' - structural array containing relevant data to Hx animal <br> In both Nx and Hx:<br> 'trial_rep' - representative trial event mark<br> 't_rep' - time<br> 'air_rep' - air onset event mark<br> 'dFF_480_rep' - GCaMP6f dF/F signal 'SIAppendix_FigureS3.mat' - Contains data related to SI Appendix Figure S3a and d<br> 'FP_US' - structural array containing relevant data to US-only trials<br> 'FP_US.condition_US' - 'Nx' or 'Hx'<br> 'FP_US.zUStrialsonly - z dF/F traces in US only trials<br> 'FP_US.CSUS_tmod - time with respect to t = 0 as U.S. onset<br> 'FP_CS' - structural array containing relevant data to CS-only trials<br> 'FP_CS.condition_CS' - 'Nx' or 'Hx'<br> 'FP_CS.zCStrialsonly - z dF/F traces in CS only trials<br> 'FP_CS.CSUS_tmod - time with respect to t = 0 as C.S. onset 'Figure 2E data.pdf' - Contains data related to Figure 2E 'Figure 2F data.pdf' - Contains data related to Figure 2F 'Figure 2H data.pdf' - Contains data related to Figure 2H 'Figure 3D data.pdf' - Contains data related to Figure 3D 'SIAppendix Figure S3 data.pdf' - Contains data related to SI Appendix Figure S3 'SIAppendix Figure S7 data.pdf' - Contains data related to SI Appendix Figure S7 'SIAppendix Figure S8 data.pdf' - Contains data related to SI Appendix Figure S8 'SIAppendix Figure S9 data.pdf' - Contains data related to SI Appendix Figure S9
本文件夹包含与论文《新生浦肯野细胞功能紊乱是损伤相关性学习缺陷的潜在机制》（Disruption of neonatal Purkinje cell function underlies injury-related learning deficits）相关的数据集。作者为Aaron Sathyanesan<sup>1</sup>*、Panagiotis Kratimenos<sup>1,2,3</sup>与Vittorio Gallo<sup>1,3</sup>*。 <sup>1</sup> 美国华盛顿特区儿童国家医院儿童国家研究所神经科学研究中心；<sup>2</sup> 新生儿科分部；<sup>3</sup> 乔治华盛顿大学医学院与健康科学学院，华盛顿特区。 * 共同通讯作者。 通讯邮箱：vgallo@childrensnational.org（Vittorio Gallo）、asathyanesan@childrensnational.org（Aaron Sathyanesan）。 ### 文件详情 1. `Figure1.mat`：包含与图1D中代表性光纤光度记录轨迹相关的数据，各字段说明如下： - `t`：记录总时长（单位：秒） - `dFF_480`：GCaMP6f的ΔF/F₀轨迹 - `dFF_405`：等吸收对照轨迹 - `USonly`、`CSonly`、`Paired`与`trials`：经分类的带时间标记的事件。 2. `Figure2_and_SIAppendix_FiguresS4-S6.mat`：包含与图2A-D及补充附录图S4-S6相关的数据，各字段说明如下： - `FP_1`：包含核心实验数据的结构体数组 - `FP_1.condition`：分组标识，取值为`Nx`或`Hx`（对应图2C-D） - `FP_1.day`：学习会话编号，可选1、2、3（对应图2C-D） - `FP_1.zCSUS`：配对学习试次中CS-US窗口内的平均z评分ΔF/F₀（对应图2G） - `FP_1.zdFF`：所有z评分标准化后的ΔF/F₀轨迹（对应图2C-D） - `FP_1.CSUS_t`：以条件刺激（CS, Conditioned Stimulus）起始为t=0的时间向量（对应图2C-D） - `FP_1.rep_cond`：代表性数据对应的分组，取值为`Nx`或`Hx`（对应图2A-B） - `FP_1.rep_day`：代表性数据对应的学习会话编号，可选1、2、3（对应图2A-B） - `FP_1.rep_dFF`：用于图2A-B的归一化GCaMP6f ΔF/F₀信号 - `FP_1.trialduration`：所有试次的时长（对应补充附录图S4） - `FP_1.missteptime`：所有试次的错误步时（对应补充附录图S5） - `FP_1.velocity`：所有试次的运动速度（对应补充附录图S6） - `FP_1.shortsteptime`：短时长试次的步时（对应补充附录图S4） - `FP_1.shortmissteptime`：所有试次的短错误步时（对应补充附录图S5） - `FP_1.longsteptime`：长时长试次的步时（对应补充附录图S4） - `FP_1.longmissteptime`：所有试次的长错误步时（对应补充附录图S5） - `FP_1.zPost`：扰动后经z评分标准化的步时（对应图2G） 3. `Figure3.mat`：包含与图3C相关的数据，各字段说明如下： - `FP_2`：包含核心实验数据的结构体数组 - `FP_2.condition`：给药分组标识，取值为`saline`（生理盐水）或`CNO`（氯氮平N-氧化物） - `FP_2.day`：学习会话编号，可选1、2、3 - `FP_2.zdFF_DRD`：所有z评分标准化后的ΔF/F₀轨迹 - `FP_2.CSUS_tmod`：以CS起始为t=0的修正时间向量 4. `SIAppendix_FigureS2.mat`：包含与补充附录图S2b-c相关的数据： - `Nx`：对应Nx组动物的实验数据结构体数组 - `Hx`：对应Hx组动物的实验数据结构体数组 两组均包含以下字段： - `trial_rep`：代表性试次的事件标记 - `t_rep`：时间向量 - `air_rep`：空气刺激起始事件标记 - `dFF_480_rep`：GCaMP6f的ΔF/F₀信号 5. `SIAppendix_FigureS3.mat`：包含与补充附录图S3a、d相关的数据： - `FP_US`：对应仅非条件刺激（US, Unconditioned Stimulus）试次的实验数据结构体数组 - `FP_US.condition_US`：分组标识，取值为`Nx`或`Hx` - `FP_US.zUStrialsonly`：仅US试次中的z评分ΔF/F轨迹 - `FP_US.CSUS_tmod`：以US起始为t=0的时间向量 - `FP_CS`：对应仅CS试次的实验数据结构体数组 - `FP_CS.condition_CS`：分组标识，取值为`Nx`或`Hx` - `FP_CS.zCStrialsonly`：仅CS试次中的z评分ΔF/F轨迹 - `FP_CS.CSUS_tmod`：以CS起始为t=0的时间向量 6. 其余数据文件说明： - `Figure 2E data.pdf`：包含与图2E相关的实验数据 - `Figure 2F data.pdf`：包含与图2F相关的实验数据 - `Figure 2H data.pdf`：包含与图2H相关的实验数据 - `Figure 3D data.pdf`：包含与图3D相关的实验数据 - `SIAppendix Figure S3 data.pdf`：包含与补充附录图S3相关的实验数据 - `SIAppendix Figure S7 data.pdf`：包含与补充附录图S7相关的实验数据 - `SIAppendix Figure S8 data.pdf`：包含与补充附录图S8相关的实验数据 - `SIAppendix Figure S9 data.pdf`：包含与补充附录图S9相关的实验数据
