Continuous single-molecule identification of full-length proteins with single-amino-acid resolution using nanopores

This database contains raw electrophysiology data. Eletrophysiology data: The electrophysiology data are grouped according to their use in the figures presented in the main text. Each folder corresponds to a specific figure and contains subfolders for each measured protein. Each subfolder contains raw traces (.abf files) of manually segmented squiggles used for analysis and figure generation. Each file includes the protein name, pore name, applied voltage. (you can find the other working conditions on the text of the manuscript).For example: MspAD90N_MBP-SQtag_-65mV_number Data from the main text: Figure 1. Ufoldase-mediated nanopore protein identification. This section contains seven folders, one for each tested protein, corresponding to the labeled letters in the figure: -(e) MBP-SQtag, (f) MNG-SQtag, (g) LBP-SQtag, (h) HitA-SQtag, (i) GBP-SQtag, (j) SpuD-SQtag, (k) MNG-SD-MBP-SQtag. Figure 2. Single amino acid substitution in protein domain. This section contains seven folders, one for each tested MBP mutant protein, corresponding to the labeled letters in the figure: (b) MBP-Y307R-SQtag, (c) MBP-N201R-SQtag, (d) MBP-F27R-SQtag, (e) MBP-R98D-SQtag, (f1) MBP-K142G-SQtag, (f2) MBP-K142E-SQtag, (f3) MBP-K142F本数据库包含原始电生理（electrophysiology）数据。 电生理数据： 电生理数据按照其用于正文章节中展示的图表的用途进行分组。每个文件夹对应一张特定图表，并包含对应每个被测蛋白质的子文件夹。每个子文件夹内包含用于分析及图表生成的经人工分段的信号波形原始轨迹（.abf格式文件）。 每个文件包含蛋白质名称、孔道名称、施加电压（其余实验条件可查阅手稿正文）。示例文件名：MspAD90N_MBP-SQtag_-65mV_number。 正文章节数据： 图1：解折叠酶（Ufoldase）介导的纳米孔蛋白质鉴定。 本部分包含七个文件夹，分别对应图中标注的字母，每一个文件夹对应一种被测蛋白质： -(e) MBP-SQtag，(f) MNG-SQtag，(g) LBP-SQtag，(h) HitA-SQtag，(i) GBP-SQtag，(j) SpuD-SQtag，(k) MNG-SD-MBP-SQtag。 图2：蛋白质结构域的单氨基酸替换。 本部分包含七个文件夹，分别对应图中标注的字母，每一个文件夹对应一种被测MBP突变体蛋白质： (b) MBP-Y307R-SQtag，(c) MBP-N201R-SQtag，(d) MBP-F27R-SQtag，(e) MBP-R98D-SQtag，(f1) MBP-K142G-SQtag，(f2) MBP-K142E-SQtag，(f3) MBP-K142F。