five

Background mutagenesis during prolonged exposure

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NIAID Data Ecosystem2026-03-14 收录
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https://www.ncbi.nlm.nih.gov/sra/ERP117926
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Assessment of accumulation of background mutations in HepG2 and MCF10A during 2 months of culture.Per cell line (MCF10A or HepG2) 3 single cell clones were established. MCF10A_SC7 and HepG2_SC1 were then subjected to a mock exposure protocol, consisting of 2 months of continuous culture followed by single cell sorting to generate '2nd generation clones' HepG2_SC1_cl1-3 and MCF10A_SC7_cl1-3. WGS was performed on all of these clones to establish the background mutagenesis levels and mutation spectrum in these cell lines
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2023-01-18
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