Analyses of gene expression data from the microglial RNA of uninfected and P. berghei-infected wild-type C57BL/6 and IFNAR knock-out mice

The C57BL/6 mouse infected with <i>Plasmodium berghei</i> ANKA is a common experimental model for cerebral malaria. In order to investigate the effect on microglial cells of Type 1 interferon signalling during experimental cerebral malaria infection, transcriptomes of naive and <i>P. berghei</i>-infected wild-type (WT) C57BL/6 and interferon-α/β receptor knock-out (IFNAR-KO) mice were generated. These transcriptomic data has been deposited at NCBI Gene Expression Omnibus (GEO) under accession number <b>GSE119650</b>.<br>This dataset presents analyses of the raw transcriptomic data available from GEO. All data are supplied either as PDF or tab-delimited text files. Files available are as follows:<br><b>PCA.pdf </b>- Principle Component Analysis of the top 500 genes showing variance across the naive and infected WT and IFNAR-KO samples.<br><br><b>wt.inf_vs_wt.naive-p01.fc2.results.txt</b> - Differential gene expression analysis of naive and infected WT microglial cells.<br><br><b>ko.inf_vs_ko.naive-p01.fc2.results.txt</b> - Differential gene expression analysis of naive and infected IFNAR-KO microglial cells.<br><br><b>ToppGene.enrichment.barplot.pdf</b> - A bar plot comparing hits to the Reactome Pathway Database derived from gene list enrichment analysis of the top 500 unique Entrez gene identifiers from each differential gene expression analysis.<br><br><b>ko_specific_treatment_effect.heatmap.pdf</b> - A heatmap indicating genes that displayed a IFNAR-KO-specific response to <i>P. berghei</i> infection versus WT microglial cells.