Data on microbial production of xylitol from high formate organosolv C5 fraction

These data files contain data concerning strains of Saccharomyces cerevisiae and Pichia kudriavzevii modified to produce xylitol from xylose, with or without additional expression of a formate dehydrogenase gene to oxidize the formic acid. The files contain data from preliminary experiments which helped to define the conditions for later experiments in bioreactor cultures (file names include the word "flasks"), as well as data from both batch and fed-batch bioreactor cultures. Bioreactor data is published in a corresponding journal article and all data included in the publication is included in this open data. However, data from related strains is also included in the open data files, where available, even though this data is not mentioned in the journal article. Data is provided for cultures grown in medium with yeast extract, peptone, glucose and xylose, in addition to the medium with formate-acetate organosolve C5 fraction. The C5 fraction generated from formic and acetic acid organosolv processes provides a good source of xylose for xylitol production, but the high formic acid content is particularly challenging for microbial processes. By expressing both xylose reductase and formate dehydrogenase genes in Saccharomyces cerevisiae or Pichia kudriavzevii, the strains tolerated C5 fraction containing up to 65 g L-1 formic acid and produced 53 to 112 g L-1 xylitol in fed batch culture. The concentration of xylose in the feed needed to be >200 g L-1 in order to achieve high (> 100 g L-1) xylitol concentrations. S. cerevisiae H4031 produced more xylitol in the C5 fraction than P. kudriavzevii H4496, although H4496 was more productive when provided pure xylose.本数据集文件收录经基因改造的酿酒酵母（Saccharomyces cerevisiae）与库德里阿兹威毕赤酵母（Pichia kudriavzevii）菌株相关数据，上述菌株可利用木糖（xylose）合成木糖醇（xylitol），部分菌株额外表达甲酸脱氢酶基因（formate dehydrogenase gene）以氧化甲酸。文件中既包含用于确立后续生物反应器培养实验条件的预实验数据（文件名含"flasks"字样，指代摇瓶培养），也涵盖分批式与补料分批式生物反应器培养的实验数据。 生物反应器培养相关数据已发表于对应期刊论文，且本开放数据集完整收录该论文中的全部数据。此外，数据集文件还收录了期刊论文未提及的相关菌株实验数据（若有留存）。本次提供的培养数据涵盖以酵母提取物、蛋白胨、葡萄糖与木糖为组分的培养基，以及含甲酸-乙酸有机溶剂法C5馏分的培养基的培养实验结果。 甲酸-乙酸有机溶剂法制备得到的C5馏分是木糖醇生产的优质木糖来源，但其高甲酸含量会对微生物发酵过程造成显著挑战。通过在酿酒酵母或库德里阿兹威毕赤酵母中同时表达木糖还原酶（xylose reductase）与甲酸脱氢酶基因，改造后的菌株可耐受含最高65 g·L⁻¹甲酸的C5馏分，并在补料分批培养中合成53~112 g·L⁻¹的木糖醇。若要获得高于100 g·L⁻¹的木糖醇终浓度，补料液中的木糖浓度需达到200 g·L⁻¹以上。在C5馏分培养基中，酿酒酵母H4031的木糖醇产量高于库德里阿兹威毕赤酵母H4496；但当以纯木糖为底物时，H4496的生产性能更优。