Supplemental Material for Liu et al., 2020
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Figure
S1 shows the sequence of the synthetized tdTomato gene with flanking homologies
to SIR2. Figure S2 illustrates the growth of clones with subtelomeric URA3
either on –ura plates or on 5-FOA plates. Figure S3 and S4 describe the
recovery of the initial expression profile after cell sorting for both low and
high Sir2-expressing cells, after 6 h and 24 h respectively. Figure S5 shows
the viability of subpopulations of the tdTomato-Tsl1-tagged strain expressing
extreme tdTomato levels similar than in the tdTomato-Sir2-tagged strain. Table
S1 lists the primers used in this study. Table S2 contains the raw data of the
silencing and viability analyses
提供机构：
GSA Journals
创建时间：
2020-07-28
