Single cell RNA seq analysis of microglia from aged wild-type, NLRP3-/-, APP/PS1 and APP/PS1.NLRP3-/- mice.

NLRP3 inflammasome activation is involved in the progression of Alzheimer's disease. To unravel the unique cell populations and clusters regulated by NLRP3 in the brain, we enriched CD11b+ cells from the brains of 18 month old wild-type, NLRP3-/-, APP/PS1 and APP/PS1.NLRP3-/- mice for single cell RNAseq. Overall design: The brains from wild-type, NLRP3-/-, APP/PS1 and APP/PS1.NLRP3-/- mice were isolated and dissociated using the Neural Tissue Dissociation kit (P) (Miltenyi Biotec), and using MACS isolation the myelin was labelled and removed magnetically before the enrichment of CD11b+ microglia following the manufacturer's guidelines (Miltenyi Biotec). Single, live, CD11b+ cells were FACS sorted at 4°C (BD FACS Aria III) into 384-well plates containing 384 primers and Mineral oil (Sigma). After sorting, plates were snap-frozen on dry ice and stored at -80°C