Three-color whole cell LLSM imaging data of ER, H2B, and Mito over 1000 time points during mitosis (H2B channel)

This dataset includes the three-color whole cell lattice light-sheet microscopy (LLSM) data of ER, H2B, and Mitochondia over 1000 time points at 6 sec intervals in a HeLa cell stably expressing calnexin-mEmerald, H2B-mCherry and Mito-Halo during mitosis (only H2B channel here due to the file size restriction, other channels can be found in the same depository with different DOI), which was used to demonstrate SiS-rDL denoising algorithm in our Nature Biotechnology paper (DOI: 10.1038/s41587-022-01471-3). This dataset can be used for non-commercial purposes with proper citations of our NBT paper.本数据集涵盖稳定表达钙联蛋白-mEmerald（calnexin-mEmerald）、组蛋白H2B-mCherry（H2B-mCherry）与线粒体靶向Halo标签蛋白（Mito-Halo）的海拉（HeLa）细胞在有丝分裂过程中，内质网（Endoplasmic Reticulum, ER）、组蛋白H2B（Histone H2B, H2B）及线粒体（Mitochondria, Mito）的三色全细胞晶格光片显微镜（Lattice Light-Sheet Microscopy, LLSM）成像数据，该数据集包含超过1000个时间点，采样间隔为6秒。受文件大小限制，本数据集仅包含组蛋白H2B通道的成像数据，其余通道的成像数据可在同一仓储库中通过不同DOI获取。本数据集曾用于验证我们发表于《自然·生物技术》（Nature Biotechnology）的论文（DOI: 10.1038/s41587-022-01471-3）中提出的SiS-rDL去噪算法。本数据集可用于非商业用途，但需正确引用上述发表于《自然·生物技术》的相关论文。