Bruno1 regulates gene expression and splicing that is necessary for proper development of the indirect flight musculature in D. melanogaster.

Building on a previous study where we examined changes in gene expression and splicing after knockdown of Bruno 1 (Bru1, Aret) in indirect flight muscle, we have now generated a CRISPR allele bru1[M3] that results in a phenotypic null. Here we examine changes in gene expression and alternative splicing in bru1[M3] mutant IFM dissected from 1 d adult flies. Overall design: Analysis of differential gene expression and exon use between control (w[1118]) IFM and bru1 mutant (bru1[M3]) IFM from 1d adult.