Isoenzyme activity in monitoring deterioration of Balfourodendron riedelianum seeds

ABSTRACT. In this study, we sought to identify efficient enzymes for use in monitoring the deterioration and loss of germination of Balfourodendron riedelianum (Engler) Engler seeds. The research was conducted at the Laboratório de Tecnologia de Sementes e Mudas of the Universidade Estadual do Oeste do Paraná, Campus Marechal Cândido Rondon, Paraná State, Brazil. Seeds were harvested at three collection sites and later submitted for processing, drying, initial characterization and storage in airtight packaging under controlled and uncontrolled storage conditions and uncontrolled. Tests were conducted at 0, 120, 240, and 360 days using a germination test, germination speed index, accelerated ageing test and the activity of peroxidase, phenylalanine ammonia-lyase and β-1,3-glucanase. The experiment was a completely randomized design. The results were subjected to regression analysis at 5% probability. Ageing was different among sample sites and between different storage conditions. The decrease in viability of seeds of Balfourodendron riedelianum (Engler) Engler was detected based on the activity of peroxidase enzymes in seeds from the Diamante do Oeste and Missal sites and the enzyme β-1,3-glucanase in seeds from the Missal site after storage periods of 0, 120, and 240 days.摘要 本研究旨在筛选可用于监测巴福尔木（Balfourodendron riedelianum (Engler) Engler）种子劣变与发芽力丧失的高效酶类。本试验于巴西巴拉那州马林沙尔·坎迪多·隆东校区的巴拉那州西部州立大学种子与苗木技术实验室开展。研究人员从三个采集点采收该树种种子，随后对其进行处理、干燥、初始特性表征，并将种子置于密闭包装内，分别采用可控与不可控储存条件进行储存。分别在贮藏第0、120、240及360天时开展检测，检测项目包括发芽试验、发芽速率指数、加速老化试验，以及过氧化物酶、苯丙氨酸解氨酶与β-1,3-葡聚糖酶的活性测定。本试验采用完全随机设计，对结果进行5%显著性水平下的回归分析。结果显示，不同采集点的种子老化程度存在差异，不同储存条件下的种子老化程度亦不相同。在贮藏0、120及240天后，可通过Diamante do Oeste与Missal采集点种子的过氧化物酶活性，以及Missal采集点种子的β-1,3-葡聚糖酶活性，检测到巴福尔木种子活力的下降。