Global Fungal Biodiversity Dataset (FunAqua)

Dataset description: The FunAqua dataset provides a global overview of fungal biodiversity in aquatic ecosystems using long-read eukaryotic metabarcoding. It includes data from 1,076 sediment samples and 1,299 filtered water samples collected across 1,452 sites in 87 countries. Sampling and processing followed standardized protocols for water and sediment. Fungal diversity was assessed by targeting the internal transcribed spacer (ITS) region, a widely accepted marker for fungal identification. High-throughput sequencing and bioinformatic analysis resulted in 50,152 fungal OTUs, representing diverse aquatic fungal communities worldwide. This dataset is a valuable resource for studies on freshwater fungal ecology, global biodiversity patterns, and ecosystem functioning, supporting research in conservation, biogeography, and environmental monitoring. FunAqua dataset composition File name File function Description Funaqua_metadata.tsv Sample data Metadata corresponding to each sample FunAqua_fungi_otu.csv OTU abundance data Table containing sequence counts of fungal OTUs in each sample FunAqua_euk_otu.csv OTU abundance data Table containing sequence counts of non-fungal OTUs in each sample FunAqua_fungi_tax.csv Taxonomy table Table with taxonomic classification data for fungal OTUs FunAqua_euk_tax.csv Taxonomy table Table with taxonomic classification data for non-fungal OTUs FunAqua_Water chemistry_Data.csv Water chemistry data Table containing results of water chemistry analyses FunAqua_sediment_chemistry.csv Sediment elemental analysis data Table containing results of sediment elemental analyses FunAqua_sediment_chemistry_references.csv Sediment elemental analysis reference data Includes analyses of calibration ranges for reference data Data reuse information: This dataset includes Data Reuse Information (DRI) tags to support equitable data sharing (Hug et al., 2025). The DRI identifies creators who prefer to be contacted before reuse: DRI: `{0009-0003-8968-6773, 0000-0003-4743-6111}` Please contact these individuals prior to reuse of the data. Water chemistry data collected by USGS found at Tangen and Bansal (2025)数据集描述： FunAqua数据集（FunAqua）借助长读长真核生物宏条形码测序技术（long-read eukaryotic metabarcoding），系统性呈现了全球水生生态系统的真菌生物多样性概况。该数据集涵盖了来自87个国家1452个采样点的1076份沉积物样本与1299份过滤水样的检测数据。 水样与沉积物样本的采集及处理均遵循标准化实验流程。真菌多样性分析以内部转录间隔区（internal transcribed spacer，ITS）为靶标区域——该区域是学界广泛认可的真菌鉴定分子标记。 通过高通量测序与生物信息学分析，共获得50152个真菌操作分类单元（Operational Taxonomic Unit，OTU），涵盖了全球范围内多样的水生真菌群落。 本数据集可为淡水真菌生态学、全球生物多样性格局及生态系统功能相关研究提供重要支撑，助力保护生物学、生物地理学与环境监测领域的科研工作。 ### FunAqua数据集组成 | 文件名 | 文件功能 | 数据描述 | | --- | --- | --- | | Funaqua_metadata.tsv | 样本元数据 | 包含各采样样本对应的配套元数据 | | FunAqua_fungi_otu.csv | OTU丰度数据 | 包含各样本中真菌OTU的序列计数表格 | | FunAqua_euk_otu.csv | OTU丰度数据 | 包含各样本中非真菌OTU的序列计数表格 | | FunAqua_fungi_tax.csv | 分类学注释表 | 包含真菌OTU的分类学鉴定信息 | | FunAqua_euk_tax.csv | 分类学注释表 | 包含非真菌OTU的分类学鉴定信息 | | FunAqua_Water chemistry_Data.csv | 水化学数据 | 包含水化学分析结果的表格 | | FunAqua_sediment_chemistry.csv | 沉积物元素分析数据 | 包含沉积物元素分析结果的表格 | | FunAqua_sediment_chemistry_references.csv | 沉积物元素分析参考数据 | 包含参考数据的校准范围分析结果 | ### 数据复用须知 本数据集包含数据复用信息（Data Reuse Information，DRI）标签，以支持公平的数据共享（Hug等人，2025）。该标签标记了希望在数据复用前先行联系的数据集创建者： DRI标识：`{0009-0003-8968-6773, 0000-0003-4743-6111}` 复用该数据集前，请务必联系上述人员。 由美国地质调查局（United States Geological Survey，USGS）采集的水化学数据可参考Tangen与Bansal（2025）的研究成果。