Additional file 4 of Holobiont nitrogen control and its potential for eutrophication resistance in an obligate photosymbiotic jellyfish

Additional file 3: Table S2. ASV abundance table detailing ASV numbers over samples, including taxonomy and representative sequences. Number of ASVs 303, of which 7 are contaminants (marked with *), core microbiome members in bold, 'symbiome' members underlined for symbiotic samples and 'apobiome' members underlined for aposymbiotic samples. Table S3. Detailed stable isotope measurements of Cassiopea xamachana and its associated Symbiodiniaceae in symbiotic-light (SymL), symbiotic-dark (SymD), aposymbiotic-light (ApoL) treatments and upon heterotrophic feeding. Table S4. Stable isotope analysis enrichment statistics. Table S5. Stable isotope labelling of Isochrysis galbana and Artemia salina. 13C and 15N enriched Isochrysis galbana algae were employed to label Artemia salina as a heterotrophic food source for C. xamachana. Table S6. OTU abundance table detailing OTU numbers over samples, including taxonomy and representative sequences. Number of OTUs 697, of which 9 are contaminants (marked with *), core microbiome members in bold, 'symbiome' members underlined for symbiotic samples and 'apobiome' members underlined for aposymbiotic samples. Table S7. Closest blastn hits considered for presumably important OTUs (including core microbiome, symbiome, and apobiome; assessed June 2020). Table S8. Closest complete genomes of the 20 most abundant ASVs based on an NCBI blastn search (assessed November 2020). Considered gene homologs are related to nitrogen cycling and transport.附加文件3：表S2。扩增子序列变异体（Amplicon Sequence Variant，ASV）丰度表，详细记录各样本中的ASV数量，同时包含分类学信息与代表性序列。本表共收录303个ASV，其中7个为污染物（以*标记）；核心微生物组成员以粗体标注，共生样本的共生微生物组（symbiome）成员以下划线标注，无共生体样本的非共生微生物组（apobiome）成员亦以下划线标注。 表S3：沙栖倒立水母（Cassiopea xamachana）及其共生虫黄藻科（Symbiodiniaceae）在共生光照（SymL）、共生黑暗（SymD）、无共生体光照（ApoL）处理及异养投喂条件下的高精度稳定同位素检测数据。 表S4：稳定同位素分析富集统计数据。 表S5：球等鞭金藻（Isochrysis galbana）与卤虫（Artemia salina）的稳定同位素标记实验数据。本研究使用经碳13（¹³C）与氮15（¹⁵N）富集的球等鞭金藻对卤虫进行标记，将其作为沙栖倒立水母（Cassiopea xamachana）的异养投喂饵料。 表S6：操作分类单元（Operational Taxonomic Unit，OTU）丰度表，详细记录各样本中的OTU数量，同时包含分类学信息与代表性序列。本表共收录697个OTU，其中9个为污染物（以*标记）；核心微生物组成员以粗体标注，共生样本的共生微生物组（symbiome）成员以下划线标注，无共生体样本的非共生微生物组（apobiome）成员亦以下划线标注。 表S7：针对预设重要OTU（涵盖核心微生物组、共生微生物组（symbiome）与非共生微生物组（apobiome））的最优blastn比对结果（2020年6月评估）。 表S8：2020年11月通过NCBI blastn检索得到的20个丰度最高ASV的最优完整基因组匹配结果。本次分析涉及的基因同源物均与氮循环及氮转运过程相关。