aBinding kinetics values derived from global curve-fitting analysis using divalent analysis model. bBinding kinetics values derived from global curve-fitting analysis using 1∶1 Langmuir binding model.
The rate constants are from the SPR experiments in Figure 3 of Alam et al. [4]. In these experiments Fab or mAb binds from solution to peptide-liposome conjugates. The best fit is obtained with a two-
The binding kinetics of these mAbs were further assessed by BLI assays, and equilibrium constant (KD) values were calculated. BLI, bio-layer interferometry; mAb, monoclonal antibody; NAb, neutralizing
Publicity graphic to accompany PLOS ONE "Kinetic Characterisation of a Single Chain Antibody against the Hormone Abscisic Acid: Comparison with Its Parental Monoclonal" by George O. Badescu, Andrew Ma
Association (Kon) and dissociation (Koff) rate constants for purified MAb DB81 were measured by surface plasmon resonance. The Kd was calculated as Koff\\Kon.
