RNA-seq of PgrCre/+Srfflox/flox and Srfflox/flox mouse uterus at gestation day 3.5

Upon conditional ablation of Srf in the Pgr-expressing cells of the mouse uterus, PgrCre/+Srff/f (SrfKO) females were infertile, displaying uterine fibrosis and failure to decidualize or support embryo implantation. RNA-seq analysis demonstrated dramatic changes in gestation day 3.5 SrfKO uterine tissues including dysregulation of cytoskeletal remodeling and growth response genes in addition to strong induction of proinflammatory cytokine signaling. These data show that SRF is key for endometrial function and fertility through regulation of inflammatory homeostasis. Four mice of each genotype were staged at gestation day 3.5 (3 days after finding vaginal plug), and RNA was isolated from uterine tissues for sequencing.