Expression data from MSCs cultured on 3D and 2D matrixes. Expression data from MSCs cultured on 3D and 2D matrixes

MSCs in vivo have a markedly different three-dimensioanal (3D) niche compared to the traditional two-dimensional (2D) culture in vitro. We used microarrays to detail the global difference of gene expression between MSCs cultured on 3D and 2D matrixes Overall design: Total RNA from each group (MSCs in 2D surface culture, MSCs in 3D collagen, chitosan, and PLGA matrixes) was analysed using gene microarrays