Raw and recalibrated phospholipidome profile of the abdomen muscle of female Northern shrimp Pandalus borealis collected in the wild from four geographic origins in the Northwest Atlantic@en

The dataset contains information on the phospholipidome profile of the abdomen muscle of female Northern shrimp Pandalus borealis collected in the wild from four geographic origins in the Northwest Atlantic: St. Lawrence Estuary (SLE; 48° 35' N, 68° 35' W, May 2018; Event \"SLE_Pandalus_2018\"), Eastern Scotian Shelf (ESS; 45° 23' N, 61° 04' W, February 2019; Event \"ESS_Pandalus_2019\"), Esquiman Channel (EC; 50° 44' N, 57°29' W, July 2019; Event \"EC_Pandalus_2019\") and Northeast Newfoundland Coast (NNC; 50° 18' N, 54° 16' W, November 2019, Event \"NNC_Pandalus_2019\"). Female shrimp were exposed for 30 days under laboratory conditions to several ocean global change scenarios, with three temperatures (low temperature of 2 °C, intermediate temperature of 6 °C, and elevated temperature of 10 °C) and pH (current pH 7.75 and low pH 7.40). This experiment aimed to determine the changes in the phospholipidome profiles of shrimp from different origins in response to different scenarios of future ocean global changes combining Ocean Warming (OW) and Ocean Acidification (OA) and low dissolved oxygen (DO). Phospholipid species were quantified in abdomen muscle of female shrimp using high-resolution HILIC-MS/MS. Several datasets are provided, containing (i) the raw data expressed as peak area, (ii) the peak area data recalibrated to the internal standards for each class of phospholipid, and (iii) the final normalised data following transformation using ordered quantile normalisation, along with (iv) the metadata associated with abdomen muscle samples. A total of 269 phospholipids were identified and quantified from 260 female shrimp, with 10 shrimp per treatment exposed sampled from two tanks per treatment (5 shrimp in each). Phospholipid peak areas determined using Xcalibur data system v3.3 (Thermo Fisher Scientific, USA). Shrimp that molted/died during respirometry experiments were excluded from the analysis. Shrimp that were exposed to low DO were removed from the dataset prior to data transformation and downstream statistical analysis, but the raw data is provided.本数据集包含西北大西洋4个地理区域野生雌性北方长额虾（Pandalus borealis）腹部肌肉的磷脂组学（phospholipidome）特征信息，采样地分别为：圣劳伦斯河口（St. Lawrence Estuary, SLE；北纬48°35′，西经68°35′，2018年5月；事件标识"SLE_Pandalus_2018"）、东斯科舍陆架（Eastern Scotian Shelf, ESS；北纬45°23′，西经61°04′，2019年2月；事件标识"ESS_Pandalus_2019"）、埃斯基曼海峡（Esquiman Channel, EC；北纬50°44′，西经57°29′，2019年7月；事件标识"EC_Pandalus_2019"）以及纽芬兰东北海岸（Northeast Newfoundland Coast, NNC；北纬50°18′，西经54°16′，2019年11月；事件标识"NNC_Pandalus_2019"）。 实验期间，将雌性虾置于实验室条件下暴露30天，模拟多种海洋全球变化情景，设置3个温度梯度（低温2℃、中温6℃、升温10℃）与2个pH梯度（当前pH 7.75、低pH 7.40）。本实验旨在解析不同地理起源的虾类，在耦合海洋变暖（Ocean Warming, OW）、海洋酸化（Ocean Acidification, OA）与低溶解氧（Dissolved Oxygen, DO）的未来海洋全球变化情景下，其磷脂组学特征的响应变化。 采用高分辨亲水相互作用色谱-串联质谱（HILIC-MS/MS）对雌性虾腹部肌肉中的磷脂类物种进行定量分析。本次提供的数据集包含四类数据：(i) 以峰面积表示的原始数据；(ii) 针对每类磷脂的内标物校准后的峰面积数据；(iii) 经有序分位数归一化变换后的最终标准化数据；以及(iv) 腹部肌肉样本的关联元数据。 本次研究从260尾雌性虾中鉴定并定量了共计269种磷脂，每个处理组设置2个重复养殖缸，每个养殖缸采样5尾虾，即每个处理组共采样10尾虾。磷脂峰面积通过Xcalibur 3.3版数据处理系统（赛默飞世尔科技，美国）测定。 在呼吸代谢实验过程中发生蜕皮或死亡的虾被排除在本次分析之外。暴露于低溶解氧环境的虾在数据变换与下游统计分析前已被从数据集中移除，但原始数据仍予以保留。