Validation of transcriptome data using quantitative and semi-quantitative real-time PCR.

Abbreviations and symbols: NS (Not significant, fold change was less than 2.0), RND (Resistance-Nodulation-Cell Division), + (represents increased gene expression in mutant strain based on increased abundance of PCR amplicon compared to PAO1), − (represents decreased gene expression in mutant strain based on decreased abundance of PCR amplicon compared to PAO1), NC (no apparent change in abundance was observed).缩略语与符号说明：NS（无显著差异，即倍数变化小于2.0），RND（耐药结节分化家族，Resistance-Nodulation-Cell Division），+（表示相较于PAO1，突变菌株的基因表达上调，判定依据为其PCR扩增产物丰度更高），−（表示相较于PAO1，突变菌株的基因表达下调，判定依据为其PCR扩增产物丰度更低），NC（未观察到丰度存在明显变化）。