Summary of the main outcomes of this study.

The development of Healthcare-Associated Infections (HAIs) represents an increasing threat to patient health. In this context, Pseudomonas aeruginosa is responsible for various HAIs, determining about 20% of the infections in hospitalized patients, which makes it one of the most effective pathogens due to its strong ability to form biofilms. Using Cu-based materials as foils on high-touch surfaces can help to prevent and mitigate P. aeruginosa contamination in biohazardous settings. However, the antibiofilm properties of Cu-based surfaces against P. aeruginosa may vary due to frequent touches combined with indoor environmental exposure. The main aim of this study is to investigate the impact of accelerated ageing, mimicking a high-touch frequency by cyclic exposure to artificial sweat solution as well as to temperature and relative humidity variations, on the efficacy of Cu-based thin foils against P. aeruginosa biofilms. Three Cu-based materials (rolled and annealed Phosphorous High-Conductivity (PHC) Cu, Cu15Zn brass, and Cu18Ni20Zn nickel silver) were evaluated. The ageing process enhanced the antibiofilm properties, due to an increment in Cu ion release: aged PHC Cu and Cu15Zn exhibited the highest Cu ion release and hence the highest biofilm inhibition (decrease in colony forming unit (CFU)) in comparison to their pristine counterpart, while aged Cu18Ni20Zn displayed the lowest biofilm formation reduction, despite showing the highest aesthetic and morphological stability. The Cu-based surface, which highlited the highest biofilm formation inhibition due to accelerated ageing, was Cu15Zn.医疗相关感染（Healthcare-Associated Infections, HAIs）的发生已对患者健康构成日益严峻的威胁。在此背景下，铜绿假单胞菌（Pseudomonas aeruginosa）可引发多种HAIs，约占住院患者感染病例的20%；因其具备极强的生物膜形成能力，该菌已成为临床最具威胁的病原菌之一。在高频接触表面应用铜基箔材，可有效预防并缓解生物危险环境中的铜绿假单胞菌污染。然而，受高频接触与室内环境暴露的共同影响，铜基表面针对铜绿假单胞菌的抗生物膜性能可能发生变化。本研究的核心目标为：探究通过循环暴露于人工汗液溶液并模拟温湿度波动以模拟高频接触场景的加速老化处理，对铜基薄箔抗铜绿假单胞菌生物膜效能的影响。本次研究共评估了三种铜基材料：轧制退火高导磷铜（Phosphorous High-Conductivity, PHC Cu）、Cu15Zn黄铜以及Cu18Ni20Zn锌白铜。研究发现，加速老化处理可通过提升铜离子释放量增强材料的抗生物膜性能：相较于原始未处理样品，老化后的PHC Cu与Cu15Zn黄铜的铜离子释放量最高，因此生物膜抑制效果（菌落形成单位（Colony Forming Unit, CFU）的减少量）也最为显著；而老化后的Cu18Ni20Zn锌白铜尽管在外观与形貌稳定性上表现最优，但生物膜形成抑制率最低。经加速老化处理后抗生物膜抑制效果最佳的铜基表面为Cu15Zn黄铜。