Viability and apoptosis measured by imaging in BRAF(V600E/D) melanoma cell lines following treatment with combinations of two compounds (viability/apoptosis dataset 1 of 2)

The dose response of human melanoma cell lines in response to Vemurafenib in combination with a second compound was measured at 72 hr to determine their effects on apoptosis and viability. Cells were treated in 3 replicates using a Hewlett-Packard (HP) D300 Digital Dispenser with 4 doses of Vemurafenib (1:3.16 dilution ratios) and 1 or 3 doses of a second compound for 72 hr. To score viability and apoptosis, a dye-based imaging assay was used. The cell-permeable DNA dye Hoechst 33342 was used to mark all nuclei, and DEVD-NucView488 Caspase-3 substrate was used to mark the nuclei of cells undergoing apoptosis (i.e., in which caspase 3 is active).