Detergent-resistance percentage (conversion rate) of <i>E. histolytica</i> trophozoites treated with treatment solution at different times.

aTreatment solution (Merck): Hydrogen peroxide 30%, cadmium 0.02 ppm, cobalt 0.02 ppm, copper 0.02 ppm, iron 0.1 ppm, nickel 0.02 ppm, lead 0.02 ppm, zinc 0.02 ppm, free Sulfuric acid 40 ppm, chlorine 0.5 ppm, phosphate 5 ppm, sulfate 2 ppm. bThree independent experiments were done in triplicates. Conversion rate: percent of cells that were resistant to 0.5% SDS. FDA: fluorescein diacetate. S.D.: Standard deviation.