Gene Expression Modifications by Temperature-Toxicants Interactions in Caenorhabditis elegans

Although organophosphorus pesticides (OP) share a common mode of action, there is increased awareness that they elicit a diverse range of gene expression responses. As yet however, there is no clear understanding of these responses and how they interact with ambient environmental conditions. In the present study, we investigated genome-wide gene expression profiles in the nematode Caenorhabditis elegans exposed to two OP, chlorpyrifos and diazinon, in single and combined treatments at different temperatures. Our results show that chlorpyrifos and diazinon induced expression of different genes and that temperature affected the response of detoxification genes to the pesticides. The analysis of transcriptional responses to a combination of chlorpyrifos and diazinon shows interactions between toxicants that affect gene expression. Furthermore, our combined analysis of the transcriptional responses to OP at different temperatures suggests that the combination of OP and high temperatures affect detoxification genes and modified the toxic levels of the pesticides.尽管有机磷农药（organophosphorus pesticides, OP）具有共同的作用模式，但学界已愈发认识到，此类农药可诱发多样化的基因表达应答反应。然而截至目前，学界尚未明确阐明这些应答反应的具体机制，以及它们与周遭环境条件之间的相互作用关系。在本研究中，我们针对不同温度条件下，分别及联合暴露于两种有机磷农药——毒死蜱（chlorpyrifos）与二嗪磷（diazinon）——的秀丽隐杆线虫（Caenorhabditis elegans），开展了全基因组基因表达谱分析。研究结果表明，毒死蜱与二嗪磷可诱导不同的基因表达，且温度会影响解毒基因对农药的应答反应。针对毒死蜱与二嗪磷联合暴露的转录应答分析显示，两种毒物之间存在相互作用，可对基因表达产生影响。此外，我们对不同温度下有机磷农药转录应答的联合分析表明，有机磷农药与高温的共同作用可影响解毒基因的表达，并改变农药的毒性水平。