Lactic Bacterium and Bacillus Sp. Biofilms Can Decrease the Viability of Salmonella Gallinarum, Salmonella Heidelberg, Campylobacter Jejuni and Methicillin Resistant Staphylococcus Aureus on Different Substrates

ABSTRACT This study aimed to evaluate the efficacy of biofilms formed by lactic acid bacteria and Bacillus sp. (BLA) in preventing and controlling the formation of wild biofilms and/or planktonic forms of Salmonella Gallinarum (SG), Salmonella Heidelberg (SH), and methicillin-resistant Staphylococcus aureus (MRSA) on different surfaces. The SH and SG viability was evaluated in polystyrene plates, wood shavings, and soil samples. Two protocols were developed to examine the use of BLA in a preventive and control application. For analysis of Campylobacter jejuni (CJ) BLA was used only preventively in a polystyrene plate. Results showed that BLA was effective in preventing the growth of SG and SH in all matrices. The effectiveness of BLA for MRSA was lower than for SG and SH. The efficiency of BLA in preventing CJ growth seems to be related to the initial CJ contamination. BLA proves to be a potential alternative to control food-borne pathogens commonly encountered in animal production and food industry.摘要 本研究旨在评估乳酸菌（lactic acid bacteria）与芽孢杆菌属（Bacillus sp.）形成的生物膜（BLA），在不同载体表面预防、控制鸡沙门氏菌（Salmonella Gallinarum, SG）、海德堡沙门氏菌（Salmonella Heidelberg, SH）以及耐甲氧西林金黄色葡萄球菌（methicillin-resistant Staphylococcus aureus, MRSA）野生型生物膜及/或浮游态菌体形成的效能。本研究在聚苯乙烯板、木屑与土壤样本中评估了SH与SG的活菌存活情况。研究开发了两套实验方案，分别用于考察BLA在预防性应用与控制场景中的使用效果。针对空肠弯曲杆菌（Campylobacter jejuni, CJ）的分析实验中，BLA仅在聚苯乙烯板中被用于预防性处理。实验结果表明，BLA可有效抑制所有受试基质中SG与SH的增殖。BLA对MRSA的抑制效果弱于其对SG与SH的作用。BLA对CJ增殖的预防效率似乎与CJ的初始污染水平相关。本研究证实，BLA可作为潜在替代方案，用于防控动物生产与食品工业中常见的食源性致病菌。