Rank correlations between pre-existing anti-Ad5 NAb titers and ELISpot, CD4+ T cell and CD8+ T cell IFN-γ activities, ELISA and Sporozoite IFA titers.

Pre-existing Ad5 NAb titers measured just prior to Ad immunization were tested for negative correlations with CSP and AMA1 activities by IFN-γ ELISpot, total IFN-γ CD4+ T cell ICS, total IFN-γ CD8+ T cell ICS, ELISA and sporozoite IFA for all volunteers (n = 15). r = rank correlation coefficient, and p = p value for the null hypothesis that the correlation is zero (two-tailed). Significant correlations are shown in bold.针对全部15名志愿者（n = 15），本研究通过干扰素γ酶联免疫斑点试验（IFN-γ ELISpot）、总干扰素γ CD4+ T细胞胞内细胞因子染色（intracellular cytokine staining, ICS）、总干扰素γ CD8+ T细胞胞内细胞因子染色（ICS）、酶联免疫吸附试验（Enzyme-Linked Immunosorbent Assay, ELISA）以及子孢子间接免疫荧光试验（indirect immunofluorescence assay, IFA），检测了受试者在Ad载体疫苗免疫前即刻测得的腺病毒5型（Ad5）预先存在的中和抗体（neutralizing antibody, NAb）滴度，与环子孢子蛋白（circumsporozoite protein, CSP）和顶膜抗原1（apical membrane antigen 1, AMA1）活性之间的负相关性。其中r为秩相关系数，p为“相关系数为0”的双侧检验原假设对应的p值。具有统计学显著性的相关结果以粗体标注。