Dataset: Rapid UPF1 depletion illuminates the temporal dynamics of the NMD-regulated human transcriptome. Boehm et al

This dataset contains the imaging source data for the manuscript titled "Rapid UPF1 depletion illuminates the temporal dynamics of the NMD-regulated human transcriptome" by Boehm et al. published in Molecular Cell in 2025.

The unprocessed and uncompressed imaging data (western blot and RT-PCR gels) for each main and supplemental figure can be found in the respective figure folder. For RT-PCR images, TIF (.tif) and raw Bio-Rad Image Lab Image Document (.scn) files are provided. For Western blots, multi-page TIFs (pages: 1. overlay, 2. raw chemiluminescence, 3. marker image, 4. final) are provided. Markers for western blots: PageRuler Prestained Protein Ladder (ThermoFisher Cat# 26617) and for RT-PCR: HyperLadder 1kb (Meridian Cat# BIO-33026).

The PDF file "Boehm et al Overview" provides an overview of all main and supplemental figure images used in this study.

本数据集为2025年发表于《Molecular Cell（分子细胞）》、Boehm等人题为"快速敲除UPF1揭示无义介导的mRNA降解（NMD）调控的人类转录组时间动态特性"的学术论文提供成像源数据。
所有主图与补充图对应的未处理、未压缩成像数据（蛋白质免疫印迹（western blot）与逆转录聚合酶链反应（RT-PCR）凝胶电泳结果）均可在对应图文件夹中获取。其中，RT-PCR成像数据包含TIF格式（.tif）文件与Bio-Rad Image Lab原始成像文档（.scn）文件；蛋白质免疫印迹数据则提供多页TIF文件，各页依次为：1. 叠加图、2. 原始化学发光图、3. 分子量标志物图、4. 最终处理图。
本研究中蛋白质免疫印迹实验所用分子量标准品为PageRuler预染色蛋白分子量标准品（PageRuler Prestained Protein Ladder，赛默飞世尔（ThermoFisher），货号：26617）；RT-PCR实验所用分子量标准品为HyperLadder 1kb（Meridian，货号：BIO-33026）。
名为"Boehm et al Overview"的PDF文件汇总了本研究所有主图与补充图的成像素材概览。