Statistical analysis R code.

Random, unbiased fragmentation of genomic DNA is necessary for next generation sequencing (NGS), yet the process of DNA fragmentation is still a bottleneck in NGS pipelines. A technology that increases the efficiency and consistency of this step will be highly desirable for both research laboratories and in clinical diagnostics. Previously, we reported the development of a novel cavitation enhancement reagent that dramatically decreases the time and acoustic energy required for genomic DNA fragmentation. The inclusion of this reagent in standard protocols facilitates highly efficient sonication enabling the use of widely available and inexpensive equipment, including water bath-based sonicators. Here, we report that cavitation enhancement facilitates parallel fragmentation of up to 96 samples of genomic DNA in a modified sonication device. The parallel processing of multiple samples significantly reduces processing time, while maintaining fragment range reproducibility and preserving DNA quality for NGS. Consequently, this system removes a key bottleneck in standard NGS pipelines and facilitates efforts toward research and personalized genomics.基因组DNA的随机无偏片段化是下一代测序（Next Generation Sequencing，NGS）的必要前提，但DNA片段化流程仍是NGS实验流程中的关键瓶颈。能够提升该步骤效率与一致性的技术，对于科研实验室与临床诊断领域均具有极高的应用价值。此前我们已报道开发出一种新型空化增强试剂，可大幅缩短基因组DNA片段化所需的时间与声能。将该试剂加入标准实验流程后，可实现高效超声破碎，从而能够使用普及度高、成本低廉的设备，包括水浴式超声破碎仪。本研究中，我们证实空化增强技术可在改装后的超声破碎设备中，实现最多96个基因组DNA样本的并行片段化。多样本并行处理可显著缩短实验时长，同时保证片段大小分布的重现性，并维持NGS测序所需的DNA质量。因此，该系统消除了标准NGS流程中的核心瓶颈，可为科研与个性化基因组学研究提供有力支撑。