TnSEQ analysis measuring fitness costs of insertions in the M. bovis AF2122/97 genome under oxidative stress

Data files for menadione-treated and untreated Tn-seq libraries from M. bovis AF2122/97 Samples: A1 treated A1_insertions.wigC1 treated C1_insertions.wigA2 treated A2_insertions.wigC2 treated C2_insertions.wigB1 untreated B1_insertions.wigD1 untreated D1_insertions.wigB2 untreated B2_insertions.wigD2 untreated D2_insertions.wig Two independent biological replicates (1 and 2) each with two technical replicates (A and C, B and D) Raw sequencing files (.fastq.gz): R1 is sequencing read, R2 is index read with molecular barcode Processed insertion files (.wig) for each sample created with custom pipeline: https://github.com/jenjane118/tnseq_pro牛分枝杆菌（M. bovis）AF2122/97经甲萘醌处理与未处理的转座子测序（Tn-seq）文库数据文件。 样本信息： A1：处理组，对应数据文件为A1_insertions.wig C1：处理组，对应数据文件为C1_insertions.wig A2：处理组，对应数据文件为A2_insertions.wig C2：处理组，对应数据文件为C2_insertions.wig B1：未处理组，对应数据文件为B1_insertions.wig D1：未处理组，对应数据文件为D1_insertions.wig B2：未处理组，对应数据文件为B2_insertions.wig D2：未处理组，对应数据文件为D2_insertions.wig 本数据集包含2组独立生物学重复（编号分别为1和2），每组均设置2个技术重复（分别为A与C、B与D）。 原始测序文件格式为.fastq.gz，其中R1为测序读段，R2为携带分子条形码的索引读段。 各样本的处理后插入位点文件（.wig格式）通过自定义分析流程生成，源码地址：https://github.com/jenjane118/tnseq_pro