Gene expression profile at single cell level of hearts of db/+ mice and db/db mice given 1% salt (HFpEF model)

Microvascular dysfunction is an important determinant in HFpEF but the role of mural cells herein is still poorly investigated. We aimed to examine the transcriptomic signature of endothelial cells and mural cells of the micro- and macrovascular niche in healthy vs. HFpEF cell RNAsequencing. We prepared a single cell suspension of cardiomyocyte-depleted and CD45+ cells depleted hearts (n=4 male hearts per genotype) and analysed it using single cell RNAsequencing