Chromatin landscape alteration uncovers multiple transcriptional circuits during memory CD8+ T cell differentiation

RNA-seq of shSox4 and vector transduced MPEC P14 cells Overall design: CD45.1+ pMKO.1 vector or CD45.1+ CD45.2+ shRNA targeting Sox4 transduced P14 were 1:1 mixed, and 1 x 10^5 cells were transferred into the CD45.2+ recipients followed by 2 x 10^5 PFU of LCMV-Arm+ infection. On D8, vector transduced MPECs were sorted as L/D-CD44+CD8+CD45.1+CD127+KLRG-,shSox4 transduced MPECs were sorted as L/D-CD44+CD8+CD45.1+CD45.2+CD127+KLRG-