Transcription factor CrzA modulate fungal development and cellulolytic gene expression in Penicillium oxalicum

The goals of this study are to compare different gene expressions for Penicillium oxalicum wild type strain (WT), CrzA deletion strain (?CrzA) and CrzA recover strain (ReCrzA) in 2% starch as carbon sources. The correlation analysis results between the various samples showed that the gene expression levels of the wild strain and the RecrzA strain were similar, and the gene expression levels between ?crzA and the wild strain were significantly different. The deletion of CrzA significantly down-regulates the expression levels of conidia pigment synthesis genes, and spore wall synthesis-related genes of Penicillium oxalicum, and also has a regulatory effect on the expression levels of genes related to the sporulation process. And the absence of CrzA can broadly down-regulate the expression level of cellulase-encoding genes, indicating that CrzA can cause a decrease in cellulase synthesis by affecting the expression of cellulase-encoding genes. The information provided by this study indicates that CrzA function is necessary for the mycelial development and cellulase activity of Penicillium oxalicum. Overall design: Examination of differential gene expressions by digital gene expression tag profiling in Penicillium oxalicum wild type strain, CrzA deletion mutant strains and CrzA recover strain in 18h in modifeid Mendal's medium with 2% starch as carbon sources. Three replicates were included for each samples.