RNA sequencing of THP-1 cells after Nuclear factor I-C knockdown

Nuclear factor I-C (NFIC) is a transcription factor involved cell growth and differentiation has been found to be upregulated in certain cancers. We also found upregulation of NFIC in Acute myleoid leukemia (AML) as compared to normal blood cells. We also foudn that its shRNA mediated knockdown inhibited growth and induced cell death in AML cell lines and primary samples. Therefore, we next became intersetde to find precise role AML, for this we performed bulk mRNA sequencing using THP-1 cells. Our sequencing resuts revealed 10,083 genes being significantly differentially expressed in NFIC knock down cells as compared to scambled control. Pathway analysis revealed upregulation of genes involved in G2M cell cycle regulation, apoptosis, DNA repair and epigenetic regulation of gene expression as well as downregulation of genes involved in cell survival such as Myc targets, MLL targets, cell adhesion, migration and invasion. All together these results suggests that NFIC promotes growth and survival of AML cells by regulation gene expression. Overall design: Human AML THP-1 cells were infected with either scrambled shRNA or shRNA against NFIC using retronectin. Four days post infection cells were sorted for GFP and processed for sequencing with three replicates for each sample.