Imaging data for Dorison et al., 2022

<strong>Imaging data for the manuscript </strong><em>"Kidney organoids generated using an allelic series of NPHS2 point variants reveal distinct intracellular Podocin mistrafficking"</em> <br> <strong>dataset.zip (29.8GB uncompressed): </strong>Raw imaris (.ims) images taken using Andor Dragonfly Spinning Disk Confocal. Organised into subfolders by subcellular stain and then order of channels. Also contains a .txt file with metadata for every image. <strong>edited_ROIs.zip (8.46GB uncompressed): </strong>Podocyte region of interest for each image as TIFF files (.tiff), manually proofread by an expert human annotator (Aude Dorison). <strong>imageJ_rollingball.zip (32.4GB uncompressed)</strong>: Relevant channels of each raw image with background subtraction (using rolling ball method with radius of 50 pixels) processed using Fiji/ImageJ as TIFF files (.tiff). This is used for downstream processing. <br> Code is available at https://github.com/KidneyRegeneration/Dorison2022/